3 reviews. Compare Anti-H2AX Antibody Products from Cell Signaling Technology from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more.

signaling and insulin resistance: evidence from mice and auditory HEI-OC1 cells. Quantitative γ-H2AX immunofluorescence method for DNA double-strand Radiation Therapy Model, Antioxidants & Redox SignalingVol.

Compare Anti-H2AX Antibody Products from Cell Signaling Technology from leading suppliers on Biocompare. View specifications, prices, citations, reviews, and more. Direct quantification of gamma H2AX by cell-based high throughput screening for evaluation of genotoxicity of pesticides in a human thyroid cell lines. Hershman JM(1), France B(2), Hon K(1), Damoiseaux R(3).

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H2AX is a variant of histone H2A, one of the histone core molecules forming the nucleosome, and is a vital component in repairing DNA damage (1-4). 1/17/2021 Phospho-Histone H2A.X (Ser139) Antibody (#2577) Datasheet Without Images Cell Signaling Technology https://www.cellsignal.com/datasheet.jsp?productId=2577&images=0&protocol=0 1/2 Pro d u ct U sa g e In fo rma ti o n A pplic at ion Dilut ion Wes t ern B lot t ing 1: 1000 Life and death fate decisions allow cells to avoid massive apoptotic death in response to genotoxic stress. Although the regulatory mechanisms and signalling pathways controlling DNA repair and apoptosis are well characterized, the precise molecular strategies that determine the ultimate choice of DNA repair and survival or apoptotic cell death remain incompletely understood. In response to DNA double strand breaks, the histone variant H2AX at the break site is phosphorylated at serine 139 by DNA damage sensor kinases such as ataxia telangiectasia-mutated, forming gamma-H2AX. This phosphorylation event is critical for sustained recruitment of other proteins to repair the break.

Here we show that inactivation of the SWI/SNF complexes and downregulation of the catalytic core subunits of the complexes both result in inefficient DNA double-strand break (DSB) repair and increased DNA damage sensitivity as well as a large defect in H2AX phosphorylation (gamma-H2AX) and nuclear focus formation after DNA damage.

H2AX decrease by chronic oxidative stress increases tumour cell genomic instability H2AX protein levels and subsequently the γ‐H2AX DNA damage signal. Rabbit Polyclonal Anti-gamma H2AX [p Ser139] Antibody DNA Double-strand Gamma-H2AX in recognition and signaling of DNA double-strand breaks in the Western Blot: MDC1 Antibody [NB100-395] - Human lung cancer cell lines. H2A histone family member X (usually abbreviated as H2AX) is a type of histone protein from cellular response to gamma radiation • spermatogenesis γH2AX is a sensitive target for looking at DSBs in cells. The γH2AX signal is a Feb 6, 2007 Interindividual difference in the gamma-H2AX signal in response to ionizing radiation and the DSB-inducing drug calicheamicin was almost 2-fold  The observed phosphorylation was specific to serine 139 as no signal was In contrast, there was no increase in γ-H2AX formation when these cells were  Signal amplification and induction of DNA damage-sensitive cell cycle checkpoints 𝛾 H2AX contribute to the recruitment of ATM to DSB sites and activation of  Using IF, this antibody was shown to bind to a non-nuclear location in Hela cells.

Mar 12, 2015 Conceptually, it is entirely plausible that damaged cells can signal to other ATR -dependent radiation-induced gamma H2AX foci in bystander 

See Abreview Se hela listan på academic.oup.com I use MCF-7 cell, treated with 0.5uM Doxorubicin 24 hours, did WB, and used two different gamma H2AX antibodies to detect ( #2577 in Cell signaling, rabbits and #05-636 in Millipore, mouse) I This post-translational modification determines the relative recruitment of either DNA repair or pro-apoptotic factors to the tail of serine phosphorylated histone H2AX (gamma-H2AX) and allows it to function as an active determinant of repair/survival versus apoptotic responses to DNA damage, revealing an additional phosphorylation-dependent mechanism that modulates survival/apoptotic decisions during mammalian organogenesis. The cells were then stained for gamma-H2AX or analyzed with the comet assay, to measure independently the levels of CLM-induced DNA strand breaks. The gamma-H2AX signal was present in all nucleated cell types examined. The gamma-H2AX signal and the comet signal were lower in neutrophils compared to monocytes and T-lymphocytes. Lyse cells by adding 1X SDS sample buffer (100 µl per well of 6-well plate or 500 µl for a 10 cm diameter plate).

See Abreview These gamma H2AX antibodies and suitable for use in Human, Mouse, Rat, Bovine, Canine, Chicken, Guinea Pig, Hamster, Porcine, Primate, Rabbit, and Sheep.
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See Abreview 2007-02-06 A hallmark of the cellular response to DNA double-strand breaks (DSBs) is histone H2AX phosphorylation in chromatin to generate gamma-H2AX. Here, we demonstrate that gamma-H2AX densities increase transiently along DNA strands as they are broken and repaired in G1 phase cells.

The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit was used as the secondary. See Abreview 2007-02-06 A hallmark of the cellular response to DNA double-strand breaks (DSBs) is histone H2AX phosphorylation in chromatin to generate gamma-H2AX.
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ab11174 at 1/1000 staining human HeLa cells by ICC/IF. These cells express a gene that causes a DNA damage response, leading to H2AX phosphorylation. The cells were paraformaldehyde fixed and blocked with BSA prior to incubation with the antibody for 45 minutes. An Alexa-Fluor ® 488 conjugated goat anti-rabbit was used as the secondary. See Abreview

Cells were treated with increasing concentrations of genotoxicants and nongenotoxicants. The treatment occurred for 1 h and, for temozolomide, for 2 h. The results suggest gamma-H2AX is a useful adjunct in diagnosis of metastatic RCC when RCC-Ma is negative and in higher grade RCC, which are often a diagnostic challenge. A nuclear pattern of staining of gamma-H2AX has a comparable sensitivity with RCC-Ma, and the interpretation is easier and more reliable.